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 ผลงานตีพิมพ์ในวารสารวิชาการLipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell densityผู้แต่ง: Polburee, P.,  Dr.Wichien Yongmanitchai, Lecturer , Honda, K., Ohashi, T., Yoshida, T., Fujiyama, K., Dr.Savitree Limtong, Professor , วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการLipid production from sugarcane top hydrolysate and crude glycerol with rhodosporidiobolus fluvialis using a two-stage batch-cultivation strategy with separate optimization of each stageผู้แต่ง:Boonyarit, J., Polburee, P., Khaenda, B., Zhao, Z.K., Dr.Savitree Limtong, Professor, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการCharacterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerolผู้แต่ง:Polburee, Pirapan, Dr.Wichien Yongmanitchai, Lecturer, Dr.Noppon Lertwattanasakul, Assistant Professor , Ohashi, Takao, Fujiyama, Kazuhito, Dr.Savitree Limtong, Professor, วารสาร: |
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การประชุมวิชาการLipid Production from Sugarcane Top Hydrolysate and Crude Glycerol with Rhodosporidiobolus fluvialis Using a Two-Stage Batch-Cultivation Strategy with Separate Optimization of Each Stageผู้แต่ง:Boonyarit, J, Polburee, P, Khaenda, B, Zhao, ZK, Dr.Savitree Limtong, Professor, การประชุมวิชาการ: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimized Production of L-phenylalanine by Fermentation Using Crude Glycerol) ผู้เขียน:ดร.อนุสิษฐ์ ธนะพิมพ์เมธา, รองศาสตราจารย์ , กล้า ธานีโต, เมธี ขำดวง, Kanoktip Packdibamrung, ดร.เพ็ญจิตร ศรีนพคุณ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCrude glycerol, a by-product of the commercial production of biodiesel, was used directly as the carbon source to produce the essential amino acid L-phenylalanine in an optimized fermentation process involving the novel recombinant bacterium Escherichia coli BL21(DE3). In developing the optimal process, a Plackett-Burman experimental design was used to identify the components of the medium that most affected the production of the cell mass and L-phenylalanine. For the specified carbon source and with ammonium sulfate as the nitrogen source, the media components that were the most influential were: KH2PO4, K2HPO4, NaCl, MgCl2, CaCl2, CoCl2, CuSO4, Na2MoO4, ZnSO4 and yeast extract. A three-level and five-factor central composite experimental design was then used to identify the optimal concentrations of the most influential components of the medium and the optimal duration of the fermentation. Under the optimal combination of conditions for biomass production, a biomass concentration of 1.23 g/L was achieved in 32.43 h. Similarly, under the optimal conditions for production of L-phenylalanine, a metabolite concentration of 1.03 g/L was achieved in 30.29 h. A high correlation between the models’ predicted responses and the actual responses achieved in independent validation experiments confirmed the validity of the models. |
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